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Fouzia Banu ¹, Nausheen Dawood ¹, Yamini Sudha Lakshmi ², S. Gopalakrishnan ³, V. Brinda ⁴

Affiliations
1 Department of Biochemistry, JBAS College for Women, Chennai, IN
2 Prof. Dhanapalan College of Arts and Science, Chennai, IN
3 PNG University of Technology, IN
4 CLRI, Chennai, IN

Abstract

The chemopreventive effect of Geraniol was evaluated in N-nitrosodiethylamine-induced experimental liver tumor in Wistar albino rats. In this study the animals were divided into four groups with 6 rats each and received treatments for 4 weeks. Group I served as control (untreated), Group II treated with N-nitrosodiethylamine (DEN) (200 mgkg, i.p.) and Phenobarbital (PB) (0.05%) in drinking water; Group III received Geraniol (250 mg/kg b.wt) in addition to DEN and Group IV treated with Geraniol only for four weeks. The administration of Geraniol in rats with DEN induced HCC rats (Group III) shows a significantly reduced level of liver serum marker (aspartate aminotransferase, alanine aminotransferase, acid phosphatase, alkaline phosphatase, lactate dehydrogenase, gammaglutamyl transpeptidase, alpha fetoprotein and 5'nucleotidase). These results are evident that Geraniol possesses protective effect against N-Nitrosodiethylamine (DEN)-induced Hepatocellular Carcinogenesis (HCC).

Keywords

DEN, Carcinogenesis, HCC, Geraniol, Marker Enzymes

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Yamini Sudha Lakshmi ¹, D. Mala ¹, S. Gopalakrishnan ², Fouzia Banu ³, V. Brindha ⁴

Affiliations
1 Deptartment of Biochemistry, Prof. Dhanapalan College, Chennai, IN
2 PNG University of Technology, PNG, IN
3 Department of Biochemistry, JBAS College, Chennai, IN
4 CLRI, Chennai, IN

Abstract

In this present study, the silver nanoparticles synthesized biologically from the medicinal plant Pithecellobium dulce were tested for its antimicrobial activity against E.coli, S.aureus, P.aeuruginosa, and C.albicans. The biologically synthesized nanoparticles exhibited satisfactory inhibitions against all tested microorganisms when used at different concentrations viz. 1 mM, 3 mM and 5 mM; 3 mM (20 mg/500 μl distilled water) offered the highest sensitivity. Such green-synthesized antibacterial agents locally destroy bacteria, without being toxic to the surrounding tissue.

Keywords

E.coli, C.albicans, P.aeuruginosa, Pithecellobium Dulce, S.aureus

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Yamini Sudha Lakshmi ¹, D. Mala ¹, S. Gopalakrishnan ², Fouzia Banu ³, V. Brindha ⁴, N. Gajendran ⁵

Affiliations
1 Dept of Biochemistry, Dept of Medical Biochemistry University of Madras, Chennai, IN
2 PNG University of Technology, PG
3 Department of Biochemistry, JBAS College, Chennai, IN
4 Tannary Department, CLRI, Adyar. Chennai, IN
5 Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai-600005, IN

Abstract

Objective: To introduce a cost effective and eco-friendly technique of preparing silver nanoparticles (Ag NP) from the medicinal plant, Pithecellobium dulce.

Methods: Ag NP of different concentration was prepared and characterized by using UV-Vis Spectrophotometer, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy, X-Ray Diffraction (XRD), and Fourier Transform Infra Red (FTIR).

Results: Studies by UV-Vis spectroscopy showed absorption spectra of silver nanoparticles at an absorbance peak at 475nm, in all the three different concentrations (1mM, 3mM and 5mM). The size of Ag NP ranged 50-70nm and appeared to be dominantly spherical (and occasionally triangular). The XRD pattern revealed that these silver nanoparticles have a crystalline nature.

Conclusion: These nanoparticles produced by green synthesis proved stable in solution and can have promising role in nano-medicine.

Keywords

Pithecellobium Dulce, Bioreduction, Silver Nanoparticles

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Ramprasath Ramakrishnan ¹, Jolius Gimbun ¹, Fahmi Samsuri ², Vigneswaran Narayanamurthy ², Natarajan Gajendran ³, Yamini Sudha Lakshmi ⁴, Denisa Stranska ⁵, Balu Ranganathan ⁶

Affiliations
1 University Malaysia Pahang, Gambang Campus, Gambang - 26300, MY
2 University Malaysia Pahang, Pekan campus, Pekan - 26600, MY
3 Department of Plant Biology and Plant Biotechnology, Presidency College, Chennai - 600005, Tamil Nadu, IN
4 Department of Medical Biochemistry, University of Madras, Chennai - 600113, Tamil Nadu, IN
5 InStar Technologies a.s., Liberec - 46007, Czech Republic
6 Palms Connect Sdn Bhd, Shah Alam - 40460, Selangor Darul Ehsan, MY

Abstract

Background/Objectives: Needleless electrospinning technology surmounts the shortcomings and bottlenecks of the conventional needle based electrospinning systems. Spinneret clogging and lower throughput in terms of productivity has been the setbacks of the conventional technology leading to fewer nanofibers based commercial products. Nanofiber based products finds a market in healthcare, energy and defence. Proof of concept leading to lab level prototype scaling up into industrial level of nanofiber production units is the focus of this paper. Methodology/Analysis: A simple search in the scopus web portal with a keyword ‘electrospinning’ resulted in 16,272 publications as of date. Same search for patent in US patent office portal resulted in 1939 granted patent documents. A comparison of years of technology time cycle (110 years), publications (16,272) and granted patents (1939) with the companies (31) working on electrospun products was made. Findings: Bubble, hollow tube, roller, wire based and slit-surface needleless electrospinning units are discussed on the basis of entrepreneurial product cycle of prototype development translating into commercial units. Nanofiber production at a mean diameter of 140 nm at commercial level using Poly (ethylene) oxide for Nano Drug Delivery Systems (NDDS) by wire based free surface electrospinning has been analysed. Novelty/Improvements: The analysis proves an entrepreneurial approach in the area of electrospinning as evidenced by number of patents match ups or exceeds publications in some region and also depicts its futuristic demand in several areas including healthcare and energy sectors.

Keywords

needleless elecrospinning; wire based; nanofibers; nano drug delivery systems; nanocarriers;

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